Objectives and competences
Pedagogical Objectives:
The curricular unit comprises the development of a laboratory project followed during the classes by the teachers, where each group will have its theme aiming at:
Understanding of various techniques of Microbiology and Molecular Biology
Consolidation of basic Microbiology techniques and their connection with Molecular Microbiology
Development of skills in problem solving and interpretation of results in a laboratory environment
Development of autonomy in the laboratory
Developing the capacity to plan and execute experimental activities
Development of the ability to compile, organize, discuss and integrate experimental results in a written work
Development of the ability to present the work orally
Expected Results:
It is expected that at the end of the UC the student will know the main techniques of Microbiology and Molecular Biology and understand the applicability of the various methodologies used in the different stages.
Teaching Methodologies
The UC consists of theoretical-practical classes, tutorial guidance and laboratory practical classes. Throughout the UC students will be in charge of applying techniques within the scope of the UC to solve something specific-applicability. The themes will be chosen by the students focusing on the studied techniques. At the end of the UC, the work is presented to the teachers and the whole class.
Laboratory classes are done in groups, but conducted by the teachers. The concept of morphological and biochemical characterization is introduced by classical microbiology techniques, pure culture and extraction of genetic material and gene research for microbial identification. Bacterial transformation.
Syllabus
Pratical classes:
Preparation of culture media, isolation of bacteria from a sample
Obtaining pure cultures. Confirmatory biochemical and morphological tests.
Amplification of the 16S gene. Identification and result integration with that obtained by conventional microbiology techniques. Basic bioinformatics a tool to treat sequencing results for species identification. Extraction and quantification of plasmid DNA. Bacterial transformation.
Tutorial Guidance – Research work aimed at solving a practical problem by applying the studied techniques based on the literature found.
Theoretical-Practical- Theoretical foundations to support practical classes. Systematic approach to microbiological techniques and Molecular Biology. Fundamentals of bioinformatics.
