Objectives and competences
- Provide the fundamentals of some instrumental methods of analysis and automation, complementing those usually addressed in the 1st cycle.
- Interpret the results and assess whether they are credible. To estimate precision, accuracy, sensitivity, and detection limit of instrumental analysis.
- Knowledge and understanding fundamentals of laboratory automation and of some instrumental methods of analysis (fluorescence, and mass spectrometry).
- Understanding the concepts and operation of the equipment corresponding to these methods.
- Planning how to monitor chemical processes using automatic analysis methods.
- Research: contributing for assessing the parameters involved in the development of new analytical methodologies.
Teaching Methodologies
Lectures, carried out with significant interaction with students. Laboratory classes with a duration of three hours, involving the manipulation of instrumental methods discussed in the lectures.
Syllabus
Lectures
- Automatic methods of analysis (batch, continuous, robotic, microfluidics)
- Molecular fluorescence spectroscopy
- Principles and practice of analysis by mass spectrometry
- Specificity tamdem MS-MS
- Chemometrics: Basics of spectrometry data processing (Principal component analysis and hierarchical cluster analysis).
Practical
T1 – Mass Spectrometry: identification of low molecular weight molecules
T2 – Mass Spectrometry: analysis of the samples volatile fraction using SPME
T3 – Study of a SIA system: determination of the alkalinity in water
T4 – Study of a FIA system: determination of the alkalinity in water
T5 – Molecular Fluorescence Spectroscopy
